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Twilight: “Though our families are fairly close due to Shining marrying Cadance and Celestia being my mentor, that’s not really an issue. I’m not biologically related to Luna and she had no role in my life when I was a foal, unlike Celestia.”
As a side note about some headcanon here, Cadance is a niece to Celestia in a similar way Blueblood is her nephew. It’s more like Celestia just took Cadance under her wing rather than a formal, on-paper sort of adoption. Similarly, Celestia likes to have some personal involvement with the descendants of Princess Platinum, which is her relation to Blueblood. All the royal unicorns of that line are her “nieces” and “nephews.”

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Background Pony #2533
The relationship itself is not really an issue. A breakup, now, that would have the potential for some powerful awkwardness.
Background Pony #EED2
Makes me wanna see a chart where there’s an arrow between Twilight and Luna saying “married”
 
Also this takes me back to that panel where Shining was like “Do you always have to outdo me?”
Background Pony #1512
Indeed, not one but two of Twilight’s familial links to Luna are non-biological. That’s a pretty reasonable degree of separation. With that said, there is still a degree of awkwardness when the same person is by some definition both your lover and your aunt.
@Thrond
Aunt in-law, too, technically.
 
@Thrond  
@The CLaUD
Somehow that makes it even more awkward.
 
Hot.
Background Pony #64EF
Compared to the royal families from real life they’re practically totally unrelated
Thrond

Indeed, not one but two of Twilight’s familial links to Luna are non-biological. That’s a pretty reasonable degree of separation. With that said, there is still a degree of awkwardness when the same person is by some definition both your lover and your aunt.
The CLaUD
Friendship, Art, and Magic (2018) - Celebrated Derpibooru's six year anniversary with friends.
Friendship, Art, and Magic (2017) - Celebrated Derpibooru's five year anniversary with friends.
Artist -

Definately NOT a bot!
We should also point out that, even is Cadence was biologically related to Celestia/Luna, the fact that Twi is only related through the marriage of a sibling, would only make it “incest” on the barest, most rudimentary of levels, and would have no impact on anything whatsoever.
Tragiclady
Not a Llama - Happy April Fools Day!
Friendship, Art, and Magic (2017) - Celebrated Derpibooru's five year anniversary with friends.

I can’t believe how confused some of those comments were. Princess Celestia and Luna are sisters, Cadance is adopted, and Twilight isn’t biologically related to those three. X3
 
Also Cadance/Cadense is hard to spell properly. Never looks right when I type it out D: